FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752537

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752537
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36807504
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG11574150.083.422011
GTGTTGG11815950.080.4442752
TGTTGGG11977000.077.800963
GTTGGGT12511700.074.0553364
TTGGGTG13942750.066.682445
TGGGTGT14054400.065.976446
GGGTGTG14845950.062.448587
TGTTGCG127500.060.6145863
GTTGCGT130950.058.9121134
GGTGTGT16034100.057.6352738
GTGTGTT16555100.055.4720549
TTGCGTG140550.055.2269255
GCGTGTG187000.043.7281077
TGCGTGT189950.042.4525456
CGTGTGT196350.040.9247938
TGTCGGG40150.033.7203833
GTGTCGG40650.033.6716462
TGTGTTT16619050.027.38750810-11
GTGTTTG17834150.027.32827810-11
TGTGTCG61100.025.7286031
Produced by FastQC (version 0.11.5)