FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752543

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752543
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43743381
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG11755500.080.945661
GTGTTGG11879850.078.2218862
TGTTGGG11983650.075.941653
GTTGGGT12637100.071.630734
TTGGGTG14128700.064.5099645
TGGGTGT14131200.064.005536
GGGTGTG14783350.061.0099077
GTTGCGT147100.060.5768664
GGTGTGT15941100.056.3979458
TTGCGTG160550.056.0234875
TGTTGCG165250.054.2268643
GTGTGTT16728200.053.8160329
GCGTGTG205100.045.9280937
TGCGTGT212800.043.872296
CGTGTGT215750.042.868718
TGTGTTT16939350.026.36303510-11
GTGTTTG19075800.025.06258810-11
GTTCGGT45700.024.193084
TGTTTGG19013200.023.1045812-13
GTGTTGC402700.023.0828722
Produced by FastQC (version 0.11.5)