FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752545

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752545
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52719678
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG15171050.080.945771
GTGTTGG15448600.077.670482
TGTTGGG15594550.075.4083253
GTTGGGT16449600.071.1003654
GTTGCGT163300.065.5496144
TTGGGTG18342900.064.2295155
TGGGTGT18279750.063.951936
GGGTGTG19169550.060.8320967
TGTTGCG179800.059.926553
TTGCGTG184800.058.4974065
GGTGTGT20645450.056.330928
GTGTGTT21666950.053.8375979
TGCGTGT237350.046.5462536
GCGTGTG243250.045.980047
CGTGTGT255550.043.1682178
TGTGTTT21826650.026.47565510-11
GTGTTTG24655850.025.14955710-11
GTTCGGT56000.024.6181014
GTGTTGC464950.024.173942
GTTTGGT15544900.023.16486712-13
Produced by FastQC (version 0.11.5)