FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752547

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752547
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52154865
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG14038200.080.960151
GTGTTGG14182100.077.998432
TGTTGGG14266150.075.678713
GTTGGGT15055150.071.277764
TTGGGTG16898750.063.9356735
TGGGTGT17078750.062.786416
GTTGCGT174550.062.277884
GGGTGTG18028500.059.3232847
TTGCGTG193800.056.1417665
GGTGTGT19353250.055.0873158
TGTTGCG198650.054.953873
GTGTGTT20255750.052.874029
GCGTGTG245950.045.819887
CGTGTGT260500.042.242678
TGCGTGT355400.031.562276
TGTGTTT20467550.025.9383810-11
GTGTTTG23043050.024.87882210-11
GTGTTGC458100.024.6783892
GTTGAGT266200.023.3754654
GTTTGGT14461000.022.98075312-13
Produced by FastQC (version 0.11.5)