FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752566

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752566
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24765546
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG4914000.078.5887761
GTGTTGG5101800.073.002682
GTTGCGT117550.070.764244
TGTTGGG5278050.068.769263
TGTTGCG123400.067.812813
TTGCGTG130250.064.499945
GTTGGGT5748800.062.7000924
TTGGGTG6608650.054.91635
TGGGTGT6807700.054.305346
GCGTGTG168400.051.811117
GGGTGTG7633300.048.6692737
TGCGTGT183300.048.2231526
CGTGTGT178750.048.142428
GTCGGGT28800.045.9058534
GGTGTGT8441400.043.507398
GTGTGTT8845300.040.4958539
GTGTCGG38400.037.0381552
TGTCGGG38100.035.2058683
GTGTTGC243250.034.6556322
TGTGTCG47450.032.0428431
Produced by FastQC (version 0.11.5)