FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005752590

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752590
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106385643
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG21968600.076.59731
GTGTTGG22803750.073.4328162
TGTTGGG23583450.069.933373
GTTGGGT24979200.065.757294
TTGGGTG27356050.060.6573455
TGGGTGT27155050.060.63096
GGGTGTG28175950.058.185577
GGTGTGT30230600.053.9904378
GTGTGTT31625350.051.3971029
GTTGCGT105650.036.824744
TGTTGCG127400.031.0008873
TGTGTTT31291600.025.86918810-11
TTGCGTG157600.024.9723955
GTGTTTG36089750.024.23579410-11
TGTTTGG36569450.022.01730712-13
GTTTGGT25736700.020.40984312-13
CGACATG315250.020.1125794
ACGACAT397250.019.3904894
GCGTGTG214750.019.1665157
GTTTGGG17206400.019.09427512-13
Produced by FastQC (version 0.11.5)