FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005752593

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752593
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73742671
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTGGGTGTGTTTGGTTGTTGTTTTACAAGGACTACTGGAACTGTCA1056580.14327932331065144No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG18358500.071.310151
GTGTTGG18916700.068.828642
TGTTGGG19514100.065.527923
GTTGGGT20686500.061.5382924
TGGGTGT21564000.059.4090926
TTGGGTG21879450.059.2911725
GGGTGTG22000000.057.882617
GGTGTGT23545250.053.8280458
GTGTGTT24756650.051.1420979
GTTGCGT77300.032.9134754
TTGCGTG96200.027.2529095
TGTTGCG94800.026.6061823
TGTGTTT24005100.026.1953810-11
GCGTGTG118450.024.2579567
AGACCGC377650.024.0380494
ACCGCTT201650.024.01293694
GTGTTTG29829700.022.60296410-11
GACCGCT279200.022.46190894
AAGACCG500500.022.00769294
CGTGTGT124950.021.7820578
Produced by FastQC (version 0.11.5)