FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005752596

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752596
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69086070
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG14243800.071.8904951
GTGTTGG14904350.068.454132
TGTTGGG15319650.065.475223
GTTGGGT16137250.061.8939134
TGGGTGT17634800.056.770956
TTGGGTG17824400.056.7048845
GGGTGTG18655900.053.408567
GGTGTGT20089850.049.405358
GTGTGTT21113700.046.8735589
GTTGCGT64250.028.941384
TGTTGCG76850.024.5569043
TGTGTTT20567600.023.95682710-11
GTGTTTG23752950.022.60894210-11
TGTTTGG24101500.020.35535212-13
TTGCGTG94750.020.2470285
GAACGAC262150.019.66961394
GTTTGGT16242200.019.20717412-13
GTTTGGG11762600.018.0690212-13
ACGACAT188150.018.05318394
TTGGTTT10481350.016.49166714-15
Produced by FastQC (version 0.11.5)