FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752602

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752602
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63029526
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG13840900.066.51041
GTGTTGG14594000.062.8663062
TGTTGGG15202050.059.213283
GTTGGGT16169550.055.4144064
TTGGGTG17841000.050.8779345
TGGGTGT17731350.050.7097436
GGGTGTG19102600.046.8115047
GGTGTGT20781550.042.827728
GTGTGTT21884250.040.540359
GTTGCGT62750.027.8091034
TGTTGCG73100.023.9400563
TTGCGTG78650.023.1154865
TGTGTTT21237000.020.8404510-11
GTGTTTG24351550.020.29975710-11
ACGACAT224750.018.80491494
CGACATG181600.018.13020194
TGTTTGG24692050.017.8427812-13
GTTTGGT16210200.017.4156912-13
GAACGAC315950.017.40777294
AACGACA287300.017.04216494
Produced by FastQC (version 0.11.5)