FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005752615

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752615
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87622508
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG18885800.078.253461
GTGTTGG19584400.075.0292
TGTTGGG20184500.071.5689543
GTTGGGT21281450.067.697984
TGGGTGT23388350.061.675376
TTGGGTG23630600.061.620795
GGGTGTG24626050.058.3331647
GGTGTGT26436100.054.051978
GTGTGTT27516200.051.5241439
GTTGCGT96700.037.9623074
GCGTTGG85200.037.5335162
CGTTGGG85350.036.8267333
TGCGTTG97400.035.757551
TGTTGCG115450.030.7711143
TTGCGTG140200.026.670815
TGTGTTT27349550.025.712110-11
GTGTTTG31062400.024.37556510-11
TGTTTGG31255000.022.30823912-13
GTTTGGT21030000.021.0118512-13
GCGTGTG200800.019.7807127
Produced by FastQC (version 0.11.5)