FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752621

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752621
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences175717
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG41800.077.003271
GTGTTGG42900.074.237432
TGTTGGG43550.071.585373
GTTGGGT46700.066.9972154
TGGGTGT50950.062.0576
TTGGGTG52100.060.9939545
GGGTGTG54250.057.822517
GGTGTGT59950.051.6636968
GTGTGTT63800.048.073729
TTGCGTG405.53554E-447.21825
GTTGCGT405.551236E-447.1911054
TGTTGCG500.001669258337.7312283
TGCGTGT600.004069754431.460746
TGTGTTT63600.023.83473410-11
GTGTTTG72600.022.69849210-11
TGTTTGG72200.020.6189212-13
GTTTGGT50050.019.87630712-13
TTGGTTT30800.018.13664814-15
TTTGGAC1350.00972798817.4781884
TTTGGTT38950.017.2943914-15
Produced by FastQC (version 0.11.5)