FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752622

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752622
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1244807
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG195800.074.0524751
GTGTTGG205100.068.897692
TGTTGGG207650.066.5077063
GTTGGGT220700.062.0256734
GTTGCGT3200.061.6148834
TTGCGTG3650.055.2846265
TGTTGCG3850.054.8858833
TTGGGTG256250.053.785955
TGGGTGT257600.053.0079356
GGGTGTG284000.048.0454567
TGCGTGT4700.044.9271056
GGTGTGT312750.043.4030278
GTGTGTT332050.040.6644979
CGTGTGT5350.036.8225868
GCGTGTG5850.034.4896557
CCTAAGG4400.024.5283816
GGAACGA2000.023.46240410-11
AGGAACG2200.022.39593110-11
GTGTTGC9700.022.2633172
TGTGTTT331150.020.1571910-11
Produced by FastQC (version 0.11.5)