FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005752625

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752625
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89151045
Sequences flagged as poor quality0
Sequence length100
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG12843900.082.01521
GTGTTGG13294750.078.966332
TGTTGGG14613100.071.497633
GTTGGGT15187350.068.752654
TGGGTGT16303750.064.241246
GGGTGTG16605300.063.0186587
TTGGGTG16695400.062.9925235
GGTGTGT17033500.061.355118
GTGTGTT17566650.059.562149
GTGTTTG19194500.029.3983510-11
TGTGTTT18380900.028.42356510-11
TGTTTGG19386200.026.79512412-13
GTTTGGT12497700.024.61329512-13
GTTTGGG8677150.024.59578712-13
TTGGGGG3610450.021.67457414-15
GTTGCGT59100.019.5262684
TTTGGGG5678300.019.4379414-15
TTTGGTG4660400.019.29600714-15
TTTGGTT10121850.019.13302214-15
TTGGGGT2281150.019.13245214-15
Produced by FastQC (version 0.11.5)