FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752653

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752653
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24346998
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG6181750.079.694731
GTGTTGG6519050.074.463772
TGTTGGG6631100.071.7849043
GTTGGGT7186050.066.022774
TGGGTGT8036600.059.429356
TTGGGTG8122650.059.1261255
GGGTGTG8853450.054.0479137
GTTGCGT47900.050.330614
GGTGTGT9561350.049.7922978
GTGTGTT9984100.047.359449
TTGCGTG57100.043.1776475
TGTTGCG57700.042.1428263
TGTCGGG29500.036.3464283
GTGTCGG33800.033.7403952
TGTGTCG48000.030.6738851
TGCGTGT95450.028.767066
CGTGTGT100000.028.2483628
GCGTGTG104050.027.6894327
TGTGTTT9768700.024.0809610-11
GTTGAGT82700.023.4367224
Produced by FastQC (version 0.11.5)