FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752664

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752664
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33213538
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTCTTCCTAAGGAACGACATGGCTACGATCCGACTTTTGTCTTCCTAA564850.17006619409230056No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGGA2400700.088.5520869
CCTAAGG2503450.088.2858058
TCCTAAG2587350.085.5527
CTTCCTA2655400.085.4589545
TTCCTAA2622600.085.334826
GTCTTCC2809650.081.199943
TCTTCCT2900950.078.106584
TGTCTTC2909950.078.052452
TGTGTTG5438500.075.404591
TTGTCTT3049800.074.098421
GTGTTGG5795150.070.8267442
TGTTGGG5893300.068.229073
GTTGGGT6361250.062.870954
CTAACGA37450.058.0657169
TGGGTGT7150350.056.1890376
TTGGGTG7283100.055.8639455
CCTAACG44300.055.2923438
GGGTGTG7832150.051.1379057
GGAACGA2110600.045.88033312-13
GGTGTGT8720200.045.6449858
Produced by FastQC (version 0.11.5)