FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752666

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752666
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24581872
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTCTTCCTAAGGAACGACATGGCTACGATCCGACTTTTGTCTTCCTAA257390.10470724117349564No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAAGG1881500.089.17598
CTAAGGA1815650.088.826499
TCCTAAG1951500.086.2541967
CTTCCTA2032750.086.11635
TTCCTAA2027500.085.265236
GTCTTCC2129700.082.883983
TGTCTTC2214500.079.596572
TCTTCCT2212100.079.241844
TTGTCTT2325100.075.533161
TGTGTTG3614700.074.8990861
GTGTTGG3831050.069.811992
CTAACGA47550.069.387349
CCTAACG53700.068.19218
TGTTGGG3896800.067.119583
GTTGGGT4218700.061.6882554
TTGGGTG4880450.054.320625
TGGGTGT4807900.054.2523086
GGGTGTG5268200.049.3493357
GGAACGA1546950.046.38530312-13
AGGAACG1670200.046.11189712-13
Produced by FastQC (version 0.11.5)