FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752700

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752700
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65860036
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG13028750.073.155341
GTGTTGG13854800.068.565962
TGTTGGG14018250.066.584823
GTTGGGT15298600.060.7152444
TGGGTGT16908500.055.002236
TTGGGTG17183950.054.810665
GGGTGTG18095400.051.2223667
GGTGTGT19735250.046.7863438
GTGTGTT20958700.043.9006049
GTTGCGT60150.023.6944434
TGTGTTT20350600.022.51797310-11
GTGTTTG23917450.021.04151510-11
TGTTGCG75300.019.5151123
GTTTGGT15308950.018.80823112-13
TGTTTGG23991550.018.80530712-13
ACGACAT334400.018.3944594
CGACATG270650.017.2442794
TTGCGTG85000.017.1006075
AACGACA411000.016.6518894
TTGGTTT8818850.016.53128814-15
Produced by FastQC (version 0.11.5)