FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752726

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752726
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38671666
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG6608900.075.81121
GTGTTGG7026200.071.095942
TGTTGGG7136150.068.7335053
GTTGGGT7689650.063.5036164
TTGGGTG8811850.056.213635
TGGGTGT8711650.056.177636
GGGTGTG9482800.051.4504477
GGTGTGT10342800.046.9062738
GTGTGTT11017250.043.711219
GTTGCGT36650.027.4825294
TGTTGCG43800.022.7621923
TGTGTTT10974350.021.86346410-11
TTGCGTG47850.021.6257725
GTGTTTG12886950.020.30764610-11
GTTTGGT7786200.018.80566612-13
TGTTTGG12934300.018.311212-13
TTGGTTT4357300.017.04340714-15
TTTGGTT5471600.016.35798514-15
ACGACAT152300.016.30886394
TGCGTGT73100.015.7076546
Produced by FastQC (version 0.11.5)