FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752728

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752728
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24978143
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG3729250.075.228441
GTGTTGG3946900.070.343422
TGTTGGG4018550.067.7515953
GTTGGGT4332050.062.4560664
TTGGGTG5030750.054.7784965
TGGGTGT4969150.054.5702556
GGGTGTG5476750.049.3387767
GGTGTGT6005200.044.752958
GTGTGTT6473400.041.561579
GTTGCGT31750.040.3660054
TGTTGCG36100.035.277883
TTGCGTG42600.029.8671655
TGCGTGT61350.022.1930436
TGTGTTT6484400.020.59418110-11
GCGTGTG70900.019.931897
GTGTTTG7669850.019.1336410-11
CGTGTGT73500.018.4528228
GTTTGGT4538650.017.72764412-13
TGTTTGG7686300.017.15821812-13
GAACGAC76200.016.14940894
Produced by FastQC (version 0.11.5)