FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752765

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752765
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42116636
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG9769250.079.368921
GTGTTGG10169750.075.781922
TGTTGGG10323450.073.442353
GTTGGGT10894650.069.3360444
TGGGTGT12124550.062.423326
TTGGGTG12238400.062.3222545
GGGTGTG12805800.058.9062427
GGTGTGT13705350.054.7895288
GTGTGTT14266950.052.056459
GTTGCGT65700.029.7030584
TTGCGTG66700.029.4160465
TGTGTTT14298500.025.72809610-11
GTGTTTG16393550.024.23886110-11
TGTTGCG87350.022.6899643
TGTTTGG16366400.022.1840812-13
TGCGTGT93700.022.1460276
GTTTGGT10707100.021.95546312-13
GCGTGTG111100.019.4546387
TTGGTTT6474450.019.25343114-15
GTTTGGG7572050.018.73828912-13
Produced by FastQC (version 0.11.5)