FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752773

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752773
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50818678
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG11634000.078.167021
GTGTTGG12327950.072.497052
TGTTGGG12525050.070.1012043
GTTGGGT13577600.064.4114764
TGGGTGT15132000.057.949626
TTGGGTG15331100.057.7771075
GTTGCGT101200.057.7514724
GGGTGTG16435500.053.231067
TGTTGCG113300.051.0579343
GGTGTGT17945750.048.543318
TTGCGTG126000.047.0208935
GTGTGTT19048200.045.844429
TGCGTGT172800.036.256396
CGTGTGT202250.030.8659428
GCGTGTG216550.030.0196087
TGTGTTT18834650.023.18426110-11
GTTCGGT40050.022.640084
GTGTTTG21541300.022.31407710-11
TCGGTGT47650.020.3190066
TGTTTGG21401450.019.9843512-13
Produced by FastQC (version 0.11.5)