FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752775

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752775
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56996234
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG12887300.078.409731
GTGTTGG13501150.074.4361652
TGTTGGG13665450.072.329013
GTTGGGT14514600.067.819894
TGGGTGT16091850.061.3901826
TTGGGTG16272750.061.1671185
GGGTGTG16965750.058.1132937
GGTGTGT18207450.053.8759738
GTGTGTT19160000.050.78029
GTTGCGT61550.039.530234
TGTTGCG75400.031.6068923
TTGCGTG82150.030.2822575
GTGTCGG53500.026.935432
TGTCGGG53850.026.2384513
TGTGTTT19210950.025.1726810-11
GTGTTTG22086300.023.6600910-11
TGTTTGG22144250.021.53491412-13
TGCGTGT127050.021.417866
GTTTGGT14484750.021.30754312-13
TGTGTCG83300.020.1399081
Produced by FastQC (version 0.11.5)