FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752789

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752789
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59039687
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG14045400.079.576281
GTGTTGG14750600.075.352072
TGTTGGG14912350.073.363343
GTTGGGT15892100.068.526274
TTGGGTG17997100.061.1466035
TGGGTGT17848400.061.12836
GGGTGTG18962400.057.3599177
GGTGTGT20167850.053.681668
GTGTGTT20916300.051.1820689
GTTGCGT66900.037.200094
TGTTGCG82750.030.965233
TTGCGTG85150.029.7271615
TGTGTTT20798050.025.52365910-11
GTGTTTG23799350.024.1239210-11
GTTTGGT14918950.022.08643312-13
TGTTTGG23773000.022.0415512-13
TGCGTGT131600.021.3451216
TTGGTTT8675100.019.63573514-15
TTTGGTT10726000.018.9518714-15
GTTTGGG11338550.018.59685112-13
Produced by FastQC (version 0.11.5)