FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752795

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752795
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55117026
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG12631800.077.791091
GTGTTGG13460950.072.569192
TGTTGGG13727350.069.850993
GTTGGGT14817100.064.4533844
TGGGTGT16296850.058.8899236
TTGGGTG16571050.058.4531335
GGGTGTG17574450.054.526337
GGTGTGT19403600.049.045268
GTGTGTT20788500.045.218199
GTTGCGT65750.035.538784
GTGTCGG60300.030.9615292
TGTCGGG58700.030.499253
TTGCGTG78150.029.9546995
TGTTGCG78900.029.7966333
TGTGTCG87500.023.5063021
TGTGTTT20651650.022.65370210-11
TCGGGTG84250.021.7478965
CGGGTGT83500.021.352996
GTGTTTG24611600.021.04917310-11
TGCGTGT121650.020.9987626
Produced by FastQC (version 0.11.5)