FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752803

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752803
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49315283
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG6445450.076.348111
GTGTTGG6871750.071.44162
TGTTGGG6986750.069.201633
GTTGGGT7394650.065.1363754
TGGGTGT8231000.058.8440176
TTGGGTG8408450.058.2406775
GGGTGTG8830000.054.6220057
GGTGTGT9820400.048.845048
GTGTGTT10786750.044.162359
CGCGTCG20500.026.54582618-19
GTTGCGT34700.022.593364
TGTGTTT11065550.021.54926310-11
GCGCGTC12950.020.55638918-19
GTGTTTG12895250.020.50573210-11
GCGTCGT17600.019.18689220-21
GCGACGA6000.018.89170820-21
TGTTTGG12917400.018.27554512-13
GTTTGGT8735000.017.96457312-13
GTTCGCG15500.017.78302616-17
TGTTGCG43150.017.7335173
Produced by FastQC (version 0.11.5)