FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752813

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752813
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59753367
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG13900750.080.183071
GTGTTGG14505450.076.352912
TGTTGGG14742200.073.966283
GTTGGGT15633550.069.462124
TGGGTGT17353250.062.6163676
TTGGGTG17512900.062.5658345
GGGTGTG18323350.059.1120957
GGTGTGT19669850.054.809038
GTGTGTT20539850.051.815619
GTTGCGT75150.041.5866434
TTGCGTG92650.034.717485
TGTTGCG94800.032.8660773
TGTGTTT20655200.025.53974210-11
TGCGTGT137600.024.6976246
GTGTTTG23237000.024.54194810-11
GTTTGGT14777500.022.97156312-13
TGTTTGG23164000.022.4625412-13
GCGTGTG162500.021.7125177
TTGGTTT8752050.020.64323614-15
CGTGTGT170900.020.6285888
Produced by FastQC (version 0.11.5)