FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005752817

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752817
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70636344
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG11833150.075.5985261
GTGTTGG13176400.067.534762
TGTTGGG13242300.066.131793
GTTGGGT14662900.059.487464
TGGGTGT16213700.054.113116
TTGGGTG16641550.053.4406785
GGGTGTG17846000.048.989517
GGTGTGT19756950.043.9711048
GTGTGTT21308300.040.246839
GTTGCGT64750.030.0267474
TGTTGCG82800.023.4363753
TTGCGTG85750.023.1989825
TGTGTTT21180850.020.25656110-11
GTGTTTG24716450.019.86832610-11
GTTTGGT15902850.017.80672612-13
TGTTTGG24420700.017.29428912-13
TTGGTTT9333850.016.09219414-15
AAGACCG160750.015.704905594
GTTTGGG11697250.015.39118312-13
TTGGGTT3159700.014.98739914-15
Produced by FastQC (version 0.11.5)