FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005752824

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752824
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89200985
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG13608350.074.407541
GTGTTGG14403450.069.093192
TGTTGGG14709650.066.1658553
GTTGGGT16008850.060.4931344
TTGGGTG18398950.053.5233655
TGGGTGT18232350.053.225976
GGGTGTG19968250.048.471517
GTTGCGT116650.044.62524
GGTGTGT22319200.043.1998568
GTGTGTT24439900.039.820449
TGTTGCG133650.039.805763
TTGCGTG149050.035.92015
TGCGTGT213750.026.0666986
GCGTGTG254600.022.0982347
CGTGTGT261050.021.0803248
TGTGTTT24486600.019.8316310-11
GTGTTTG28933000.018.74008610-11
GTTTGGT18473750.016.83592612-13
GAACGAC280000.016.65794894
TGTTTGG28951550.016.55553812-13
Produced by FastQC (version 0.11.5)