FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752828

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752828
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58876877
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG12383500.052.728591
GTGTTGG12924700.050.4503172
TGTTGGG13121550.049.059453
GTTGGGT13686850.046.8348054
TGGGTGT14881650.043.4367876
TTGGGTG15252700.042.935185
GGGTGTG15572350.041.233057
GGTGTGT17856150.035.7998858
GTGTGTT19572900.032.4823849
TTGGTTT9849600.024.737473
TGGTTTT8474850.024.4613384
TTTGGTT12088350.022.6544652
GGTTTTT8550350.020.5338865
GTTTGGT15524900.019.7217061
GAACGAC70950.017.26727194
TGTGTTT18580150.016.95382310-11
GTGTTTG21588950.015.93425710-11
TGTTTGG21640400.015.1828622
TGGTTTG2499000.014.2046334
GTTTTTT11022450.013.9199076
Produced by FastQC (version 0.11.5)