FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005752853

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752853
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107800393
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG27466550.083.407421
GTGTTGG29095700.078.771512
TGTTGGG29700000.077.050243
GTTGGGT30408850.075.1653064
GGGTGTG32370250.070.544727
TGGGTGT32562100.070.227666
TTGGGTG32994950.069.463675
GTGTGTT33394100.068.213549
GGTGTGT33458250.068.110598
CGGGTCG287800.036.4312483
GTGTTTG34607100.035.7687710-11
TGTGTTT33922300.033.8315210-11
TGTTTGG34218200.033.43658412-13
GTTTGGG17520400.032.7184112-13
GTTTGGT18047450.032.51481212-13
GGTCGGA199450.028.9103375
TTGGGGT4829550.028.22413614-15
TTGGGTT6599250.027.92861414-15
TTTGGTG9565900.027.7706214-15
TTTGGGT9259550.027.45701614-15
Produced by FastQC (version 0.11.5)