FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752864

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752864
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3393039
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAGCCAAGCGGTCTTAGGAAGACAACCTTCCTGGTCAACTCCTTGGCT135330.39884599027597384No Hit
GTGAGGCAAGCGGTCTTAGGAAGACAATTGATAGATTCAACTCCTTGGCT85080.25074866513470667No Hit
AGTCGGAGGCCAAGCGGTCTTAGGAAGACAATTGATAGATTCAACTCCTT84610.2493634762229376No Hit
GTGAGGCAAGCGGTCTTAGGAAGACAACCTTCCTGGTCAACTCCTTGGCT77240.22764253520221844No Hit
GTGAGCCAAGCGGTCTTAGGAAGACAATTGATAGATTCAACTCCTTGGCT77220.22758359099320696No Hit
GTGGGCCAAGCGGTCTTAGGAAGACAACCTTCCTGGTCAACTCCTTGGCT54480.16056402534718875No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTTGG266900.081.8095551
TGTTGCG73600.079.264913
GCGTGTC8150.075.0253457
GGCAAGC66100.074.696445
TCGGACG2150.074.460843
GTTGCGT83700.072.369694
GTGAGGC48550.070.2177661
CGGACGC2450.067.3412254
CGACGCC353.6089714E-667.240025
GAGGCAA62400.066.8554153
CTTGCGT7800.066.823311
GAGCCAA77800.066.815463
AGGCAAG63450.066.715044
GAGTTGG45550.066.393492
AGTTGCG3950.065.5621263
AGTTGGG42750.065.093683
GGGCCAA35200.065.010133
CGCAACG800.064.718525
GCAAGCG80400.064.563466
TGTGTTG921100.064.509051
Produced by FastQC (version 0.11.5)