FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752865

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752865
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3393039
Sequences flagged as poor quality0
Sequence length100
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGGGT399150.082.008293
AATTGGG75900.081.387652
CTCTTGG127450.081.0969541
CACTTGG112800.080.149811
TCTTGGG194850.079.798222
ACTTGGG220650.079.191182
AGTTGGG482250.075.758882
ATTGGGT119550.073.954153
GAATTGG48050.073.622031
GACTTGG103450.072.291581
GCGAACG4400.071.417315
AAGTTGG69700.070.139851
AGCGAAG30350.070.109224
GAGTTGG392950.069.178491
CAGTTGG100800.068.979111
GACAACG14250.068.4588855
AACGAGT25150.067.714388
CGAAGGA23250.067.450316
GAGCGAA33200.066.1162953
AACTTGG45200.064.977911
Produced by FastQC (version 0.11.5)