FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752866

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752866
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3592159
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAGCCAAGCGGTCTTAGGAAGACAACCTTCCTGGTCAACTCCTTGGCT148200.4125652567160863No Hit
GTGAGGCAAGCGGTCTTAGGAAGACAATTGATAGATTCAACTCCTTGGCT97810.2722875017503401No Hit
GTGAGCCAAGCGGTCTTAGGAAGACAATTGATAGATTCAACTCCTTGGCT92390.2571990827800217No Hit
GTGAGGCAAGCGGTCTTAGGAAGACAACCTTCCTGGTCAACTCCTTGGCT86990.242166340632472No Hit
AGTCGGAGGCCAAGCGGTCTTAGGAAGACAACCTTCCTGGTCAACTCCTT64330.17908450043553195No Hit
GTGGGCCAAGCGGTCTTAGGAAGACAACCTTCCTGGTCAACTCCTTGGCT58960.1641352735221353No Hit
GTGGGCCAAGCGGTCTTAGGAAGACAATTGATAGATTCAACTCCTTGGCT38970.10848628916481705No Hit
GTGAGCAAGCGGTCTTAGGAAGACAACCTTCCTGGTCAACTCCTTGGCTC37470.10431052745716433No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGACG1550.085.019523
CTGTTGG297900.081.945611
TGTTGCG78850.080.101963
GGCAAGC69250.074.378715
CGGACGC1850.073.861764
GTGAGGC52350.071.995971
GTTGCGT91550.071.643084
GCGTGTC9450.070.639637
TCTTGGG80850.068.0372242
GGGCCAA36000.066.282343
GTCTTGG59250.066.146591
GAGGCAA65800.065.947363
GCAAGCG83050.065.241366
CTTGCGT8100.064.899651
GAGCCAA82750.064.894813
GTGTTGC102150.064.804482
AGGCAAG67050.064.370974
GTTCGGT10600.062.982982
GTGGGCC29700.061.949661
GGCTGTG244050.061.9256556
Produced by FastQC (version 0.11.5)