FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752869

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752869
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5178589
Sequences flagged as poor quality0
Sequence length100
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGGGT709500.084.132253
CACTTGG215950.083.574151
ACTTGGG410100.081.916232
AATTGGG121950.081.400892
CTCTTGG209700.081.362031
TCTTGGG327050.080.4295352
GACTTGG205300.078.850991
AGTTGGG772250.077.9576952
ATTGGGT187050.075.2940143
GAATTGG87750.073.856171
CAGTTGG174550.072.374781
TGTGTTC386700.071.753169
CAATTGG43800.071.409331
CCATCGA8450.070.349936
ATGCGTG41700.069.0906142
TGAGCGA43150.067.420322
AGCGAAG38550.066.475234
AACGACT14550.065.092938
AACGAGT31700.064.982568
CATCGAC9600.064.6774447
Produced by FastQC (version 0.11.5)