FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752871

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752871
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15227699
Sequences flagged as poor quality0
Sequence length100
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTTGG1213350.088.330911
TCTTGGG2043150.087.878512
CTTGGGT2262200.085.213373
GTCTTGG998300.078.655161
ATTGGGT519400.076.623973
TATTGGG388850.075.3832
CTATTGG153050.070.9075851
GTATTGG266550.069.378211
TCTTGCG97650.067.980922
AACGAGT97700.067.380618
TGAGCGA134750.065.2914352
TTGAGTG660150.064.422412
AATTGGG144850.064.167122
TGTGTTC909750.064.136969
GTGTGTC1655900.064.000368
CAACGAC46400.063.4913067
CATCGAC28050.063.1840177
AACGACT44550.062.828178
CATCGAG38200.062.8068057
CAACGAG106750.061.9942637
Produced by FastQC (version 0.11.5)