FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752872

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752872
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1091630
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCGGAGGCCAAGCGGTCTTAGGAAGACAAGGTAGAGAGTCAACTCCTT70880.6493042514405064No Hit
GTGAGGCAAGCGGTCTTAGGAAGACAACACTTAGCTACAACTCCTTGGCT28440.26052783452268624No Hit
GTGAGCCAAGCGGTCTTAGGAAGACAACACTTAGCTACAACTCCTTGGCT24390.22342735175837966No Hit
GTGAGCCAAGCGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGCT23640.21655689198721179No Hit
GTGAGGCAAGCGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGCT21870.20034260692725556No Hit
GTGGGCCAAGCGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGCT18600.17038740232496358No Hit
GTGGGCCAAGCGGTCTTAGGAAGACAACACTTAGCTACAACTCCTTGGCT16250.14885996170863755No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCC156.8588054E-494.148985
ACGGCGA353.5470293E-1094.135967
TGTTGCG76350.089.915883
GTGTTGC83400.086.43862
GTTGCGT82650.085.941754
TCGGACG1950.082.1542743
TTGCGTG87550.081.083185
CGGACGC2050.080.419234
TGCGTGT90950.079.8008046
AGTTGCG3350.077.3577353
GCGTGTG87050.076.5091257
GCGTGTC7950.076.3744667
GTTGCCT24150.075.8714144
CGTGTCT8100.074.811958
CGTGTGT90750.073.141168
CGGCGAC452.6429916E-973.072148
ACTCGGA2850.071.0312041
TTGCCTG26150.070.566735
TCTGTTG51750.070.504521
CTCGGAG2350.070.220922
Produced by FastQC (version 0.11.5)