FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752875

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752875
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7224007
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAGCCAAGGAGTTGTAGCTAAGTGTTGTCTTCCTAAGACCGCTTGGCC115060.15927448575285158No Hit
GTGAGACAAGGAGTTGTAGCTAAGTGTTGTCTTCCTAAGACCGCTTGGCC89890.12443232682360357No Hit
GTGAGCCAAGGAGTTGTACAAAAGGATTGTCTTCCTAAGACCGCTTGGCC87110.12058404705311056No Hit
GTGAGCCAAGGAGTTGGGTCTAGTCATTGTCTTCCTAAGACCGCTTGGCC72910.10092736621102388No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGATC37850.087.948093
AACCGAT33900.084.4938662
CGGAGTT61850.080.232949
CACGGAG60850.078.6034557
TCTTGGG312050.078.067912
GACAAGG181650.077.3710865
AAACCGA48800.077.3302461
GTGAGCG59000.076.498021
CGAAGGA50500.076.3520056
TGAGCGA61250.076.243552
CCACGGA32300.074.498686
GACACGG30700.072.365415
ACCGGTC7450.072.354953
ACAAGGA198950.072.0226066
GTGAGAC201500.071.4053341
ACGGAGT73000.071.046868
GCCACGG33050.070.946395
ACCGACC10000.070.454053
AGACAAG216950.069.4880754
GTCTTGG253650.069.3918841
Produced by FastQC (version 0.11.5)