FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752876

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752876
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4601701
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCGGAGGCCAAGCGGTCTTAGGAAGACAACACTTAGCTACAACTCCTT271160.5892603626354689No Hit
GTGAGGCAAGCGGTCTTAGGAAGACAACACTTAGCTACAACTCCTTGGCT134230.29169648353945643No Hit
GTGAGCCAAGCGGTCTTAGGAAGACAACACTTAGCTACAACTCCTTGGCT123340.26803132146134656No Hit
GTGAGCCAAGCGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGCT117460.25525343780484655No Hit
GTGAGGCAAGCGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGCT114730.24932084896432863No Hit
GTGGGCCAAGCGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGCT100980.2194405938152001No Hit
GTGGGCCAAGCGGTCTTAGGAAGACAACACTTAGCTACAACTCCTTGGCT79230.17217546294294217No Hit
GTGAGCAAGCGGTCTTAGGAAGACAATCCTTTTGTACAACTCCTTGGCTC56810.12345434872887222No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTGCG295550.089.34433
CGGACGC12250.088.441974
TCGGACG11800.088.249033
GTTGCGT320450.085.2137454
GTGTTGC326550.083.7882162
TTGCGTG333400.081.8907245
GCGTGTC28700.081.365177
AGTTGCG12800.081.3545763
TGCGTGT350950.080.151926
GGACGCC13100.079.413315
GTCGGAC9800.078.9037862
GACGCCA13950.078.282086
CGCCAAG13350.078.148118
ACGCCAA13750.078.067617
GCGTGTG343750.075.4653557
GTTGCCT92150.074.018434
CGTGTCT31900.073.217098
ACTCGGA13150.073.0234761
CGTGTGT355650.072.913118
GATGCGT11050.072.0428854