FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752880

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752880
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3107501
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTGCG141750.089.40423
AGTTGCG7950.082.994843
GTGTTGC165550.081.63372
GCGTGTC14150.077.843477
CTTGCGT10600.075.961511
GTTGCGT176250.075.065184
TTGCGTC9950.074.878382
TCGGTCT7800.071.882464
TTCGGTC7150.071.188183
TTGCGTG186550.070.737035
TCTGTTG102250.070.642391
TGTGTTG936000.070.434681
GATGCGT5700.070.261054
TGCGTGT195300.069.5674446
TGCGTCT10800.068.9482963
CGTGTCT15900.068.843818
GTCTTGG47700.068.212351
GTTGCCT50150.068.020254
GAGTTGG42600.064.754322
CTTCGGT5100.063.7971652
Produced by FastQC (version 0.11.5)