FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752881

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752881
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3107501
Sequences flagged as poor quality0
Sequence length100
%GC39

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTGGG130350.076.809082
GTCTTGG105050.075.473341
CTTGGGT184750.074.468073
AGTTGGG203700.070.291742
GAGTTGG221900.067.5983351
ACTTGGG58500.066.767222
CTCTTGG68500.066.6011
GACTTGG53950.065.102091
TGAGCGA7400.064.306422
CGTGTTG40200.062.892051
GTGAGCG7850.062.37511
TCCGTTG5550.061.926631
CTTTCGT15300.061.851781
GATTCGT5800.061.6926161
GAGCGAA7400.061.123923
CTGTTTG157700.060.692765
GCTCGGT4150.060.171722
TGGCTGT93350.059.7296145
TGCTCGG7500.059.0084421
GCGAAGG6700.058.437265
Produced by FastQC (version 0.11.5)