FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752882

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752882
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5930959
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTGCG290300.089.987343
GTGTTGC333750.082.771512
GCGTGTC28200.079.956597
TTGCGTC22900.079.076692
GATGCGT11400.078.108894
AGTTGCG14150.077.936883
CTTGCGT25450.077.333351
TGCGTCT24000.076.191073
CGGACGC1750.075.381284
GTTGCGT363900.074.495724
TTCGGTC12650.073.766343
TCGGTCT14400.070.997124
TTGCGTG382350.070.8240665
TCTGTTG210600.070.693881
TGCGTGT399150.070.136556
TGTGTTG1870050.070.060931
GTCTTGG104000.069.992971
GTTGCCT106800.069.920024
CGTGTCT32400.069.593778
TGATGCG11300.067.564743
Produced by FastQC (version 0.11.5)