FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752883

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752883
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5930959
Sequences flagged as poor quality0
Sequence length100
%GC39

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTGGG570650.086.4242
GTCTTGG450200.085.0045241
CTTGGGT647100.082.905593
TCCGTTG34600.078.789131
CTCTTGG184400.074.148021
TGCGTTG52850.073.586631
CCGTTGG29500.071.476892
GGCTCTG119300.067.6842351
CTTTCGT36250.067.409831
TGCTCGG18300.066.893831
GGCTGTG230400.066.639571
TCCTCGG15900.066.626851
CTCGGTG20300.065.06423
CTCTGTT228850.064.702423
GCTCGGT11600.064.6575552
CTGTTTG303000.064.227535
TGAGCGA18700.063.5680772
GCTGTGT276000.062.724492
CGCTCTG30400.062.1061551
GCTCTGT166000.061.3798452
Produced by FastQC (version 0.11.5)