FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752884

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752884
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8704690
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTGCG413000.089.486313
GTGTTGC481300.081.672882
GCGTGTC42550.077.892127
AGTTGCG19200.077.810433
CTTGCGT34750.075.074231
GTTGCGT515100.074.285214
TTCGGTC17650.073.963063
TTGCGTC31500.072.754822
TCGGTCT20150.072.4888154
TGCGTCT32700.070.908313
TTGCGTG542700.070.405555
TGTGTTG2640700.069.63131
GATGCGT18950.069.371224
TGCGTGT570500.069.249826
TCTGTTG288550.069.08171
GTCTTGG141650.068.849271
GTTGCCT144550.068.419324
GTTCGGT34500.067.248422
TGATGCG17450.066.97863
CGTGTCT49400.066.859268
Produced by FastQC (version 0.11.5)