FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752885

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752885
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8704690
Sequences flagged as poor quality0
Sequence length100
%GC39

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTGGG399050.079.277312
CTTGGGT586350.078.108453
GTCTTGG305800.074.78911
ACTTGGG201450.071.943292
AGTTGGG630400.069.763142
GACTTGG166800.066.453531
CGCTCTG46600.066.387631
TGCTCGG22150.066.1141361
GAGTTGG638850.066.078331
CTGTTTG475550.065.83995
GCTCGGT14650.065.329912
CTCTTGG182250.065.160621
GATTCGT20350.064.3262251
TGAGCGA23550.063.863612
TGCGTTG54100.063.7120361
CTTTCGT46100.062.9198151
GGCTCTG164200.062.4586221
GTGAGCG23950.061.538441
TGTGTTC385800.061.4406979
GCGTTGG43050.060.4532472
Produced by FastQC (version 0.11.5)