FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752889

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752889
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7091321
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAGCCAAGGAGTTGATTTATGACATTGTCTTCCTAAGACCGCTTGGCC122550.17281688418843258No Hit
GTGAGCCAAGGAGTTGATTTATGACATTGTCTTCCTAAGACCGCTTGGCT92730.13076548078982744No Hit
GTGAGCCAAGGAGTTGCCTTAATTAATTGTCTTCCTAAGACCGCTTGGCC82090.11576122417811857No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGATC25750.086.302963
CGGAGTT96400.085.5904549
CACGGAG94950.084.9836047
CCACGGA75850.084.686296
TCTTGGG418300.083.33442
TGAGCGA80150.081.7131652
ACGGAGT107250.081.494788
AACCGAT23850.081.2321242
GCCACGG56250.081.0096365
CGAAGGA70200.080.5030446
CTTGGGT428950.080.1149143
GTGAGCG77550.079.26891
AGCCACG61850.079.1114964
AAACCGA37750.077.86491
AGCGAAG69250.077.764474
GAGCGAA66500.077.074863
GTCTTGG330600.074.306291
GCGAAGG68800.073.599255
CTCTTGG164600.072.91951
AACGAGT36200.072.891588
Produced by FastQC (version 0.11.5)