FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752891

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752891
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3667968
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAGCCAAGGAGTTGATTTATGACATTGTCTTCCTAAGACCGCTTGGCC71470.19484902812674484No Hit
GTGAGCCAAGGAGTTGATTTATGACATTGTCTTCCTAAGACCGCTTGGCT55960.15256403545505304No Hit
GTGAGCCAAGGAGTTGATTTATGACATTGTCTTCCTAAGACCGCTTGCCT37020.10092781616415411No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGATC23800.089.385173
AACCGAT17200.085.046152
ACCGACC8750.084.824533
TCTTGGG186650.081.783882
AAACCGA26750.081.132461
TGAGCGA33400.079.3983152
CGAAGGA32800.079.121136
CTTGGGT204100.077.710443
CTCTTGG114050.077.520711
AACCGAC6700.076.062462
GTGAGCG33500.075.465241
CACCGAT7650.074.635482
CTGAGCC85950.074.437371
ACAAGGA114600.074.3003546
AGCGAAG34550.073.809524
AACGAGT28800.072.570868
GAGCGAA33450.072.360863
GACAAGG78050.072.201775
ACCGGTC3800.072.15633
ACGAGTT30800.072.1472559
Produced by FastQC (version 0.11.5)