FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752896

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752896
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences980486
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCGGAGGCCAAGCGGTCTTAGGAAGACAATTGATAGATTCAACTCCTT25110.2560974863486067No Hit
AGACGGAGGCCAAGCGGTCTTAGGAAGACAATTGATAGATTCAACTCCTT16970.17307743302811054No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAGGG107850.086.5423362
GTGTAGG114700.086.27441
GTAGGGT189000.083.999513
GAGTAGG82700.083.538761
AGTAGGG79550.083.064342
TAGGGTG198200.082.995074
AGGGTGT222700.077.4542545
GACGCCA800.076.338786
GATGCGT4800.074.4575044
GTAGGCT14750.073.328363
TGTTGCG9450.073.151233
GAGTTGG101600.072.48251
AGATGCG2150.072.179123
AGTTGGG94450.071.801572
GTTGGCT56650.071.223533
TGATGCG3000.070.53873
GTTGCGT11550.070.4369054
AGTTGCG1950.069.935793
GGCTGTG80750.069.812086
GCTGTGT83500.068.796327
Produced by FastQC (version 0.11.5)