FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752897

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752897
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences980486
Sequences flagged as poor quality0
Sequence length100
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAGCCAAGGAGTTGGGGAAACATGTTGTCTTCCTAAGACCGCTTGGCC33700.34370710035635393No Hit
GTGAGCCAAGGAGTTGAATCTATCAATTGTCTTCCTAAGACCGCTTGGCC11600.11830867549358175No Hit
GTGAGCCAAGGAGTTGACCAGGAAGGTTGTCTTCCTAAGACCGCTTGGCC11450.11677882193116475No Hit
GTGAGACAAGGAGTTGGGGAAACATGTTGTCTTCCTAAGACCGCTTGGCC11340.11565692931872561No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGAC2350.086.1386642
ACCGACC2400.084.748893
TGAGCGA5550.082.276592
GACAAGG24250.081.7295465
CGAAGGA5450.080.816776
AGCGAAG6050.079.703094
CCGACCC2450.077.1834644
ACAAGGA26800.077.048956
TGGCTGT58150.077.033055
AAACCGA3300.076.886991
AGACAAG29000.076.61794
GCGAAGG5750.076.505385
CTCTTGG31700.075.8898541
TTGGCTG59350.075.2733844
GAGACAA27650.075.272163
GTATTGG15400.075.056351
CGACCCC2150.074.8027045
GTGAGCG6350.074.734421
GAGCGAA6100.074.442033
TCTTGGG48150.073.8156052
Produced by FastQC (version 0.11.5)