FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752898

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752898
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences862718
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCGGAGGCCAAGCGGTCTTAGGAAGACAACCTTCCTGGTCAACTCCTT31210.36176363539418444No Hit
AGACGGAGGCCAAGCGGTCTTAGGAAGACAACCTTCCTGGTCAACTCCTT18190.21084525882153843No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGACG254.8931906E-794.086743
GTGTAGG98250.086.033341
TGTAGGG92250.085.826522
GTAGGGT158050.083.6392753
AGTAGGG64850.083.374592
GAGTAGG69250.082.708881
TAGGGTG166050.082.574824
GTAGGCT14850.077.613643
AGGGTGT189000.076.231265
GATGCGT4750.075.2606354
TGTTGCG9200.073.121763
ACGGAGG4800.072.525193
GAGTTGG89500.072.1326451
TGATGCG2950.071.761073
AGTTGGG85350.071.171292
TGTAGGC10950.070.416682
TGAGTTG23100.069.5636441
GGCTGTG75000.069.472556
GTTGGCT50700.069.312423
ATGCGTG5300.069.2255865
Produced by FastQC (version 0.11.5)