FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752900

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752900
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1372653
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCGGAGGCCAAGCGGTCTTAGGAAGACAAAATATCTCTCCAACTCCTT38580.28106156472174687No Hit
AGACGGAGGCCAAGCGGTCTTAGGAAGACAAAATATCTCTCCAACTCCTT25560.1862087504999443No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGG162100.086.32721
TGTAGGG151950.085.988852
GTAGGGT265400.084.412523
AGTAGGG112950.083.3891452
GAGTAGG119900.083.331361
TAGGGTG279700.083.019254
AGTTGCG3900.079.582093
AGGGTGT313800.077.744065
GTAGGCT25200.076.510213
GATGCGT6100.076.317754
TGTTGCG15150.075.42753
GTTGCGT20000.072.652184
GAGTTGG145700.072.508691
AGTTGGG135800.072.2305152
GGCTGTG119050.072.065386
GCTGTGT120600.071.985757
ACGGAGG7350.071.658333
GTTGGCT83400.070.989753
TAGGCTG30050.070.418744
TTGGCTG103700.069.289084
Produced by FastQC (version 0.11.5)